Measurement of Low Molecular Weight Tannins : Indicators of Methanogenic Toxic Tannins

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概要

• 論文の詳細を見る

• The effectiveness of several low molecular weight(MW)tannin measurement methods for indicating the tanmin toxisity to methan bacteria was evaluated. The methanogenic toxicity of the low and high MW tannins from autoxidized bark extracts was studied by selective removal of MW fractions from the extract with active carbon adsorption and calcium precipitation treatments. The toxicity of the low MW tannin fraction and the nontoxicity of the high MW tannin fraction were demonstrated. The low MW tannin concentration, measured by HPLC and a method based on the loss of tannins by treatment with granular active carbon(AC), had a very close relationship with the methanogenic toxicity, but a poor relationship was found with the total tannin concentration. The low MW tannins detected by the HPLC and AC methods had similar peak area positions in HPLC chromatograms as the tannins that were adsorbed by polyamide(trisacyl GF05)gel beads. These gel beads have an exclusion limit of 3000g・mol^<-1>, indicating that this is the approximate MW boundary between toxic and non-toxic tannins.

• 公益社団法人日本生物工学会の論文
• 1990-03-25

著者

• Lettinga Gatze

  Deparment Of Environmental Technology Wageningen Agricultural University

• Lettinga Gatze

  Department Of Water Pollution Control Wageningen Agricultural University

• HABETS Leo

  PAQUES B.V. 環境技術担当

• Habets L

  Paques B.v. Nlt

• FIELD JAMES

  Department of Water Pollution Control, Wageningen Agricultural University, (Present address) Departm

• Field James

  Department Of Water Pollution Control Wageningen Agricultural University (present Address) Departmen

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• Measurement of Low Molecular Weight Tannins : Indicators of Methanogenic Toxic Tannins

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