Stimulatory Effects of Certain Amino Compounds, Antibiotics, and Polyamines on Production of Alkaline Phosphatase by Aeromonas liquefaciens
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概要
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Certain L- and D-amino acids, amino compounds, antibiotics, and polyamines increased production of total alkaline phosphatase by Aeromonas liquefacieens. The maximum production increased 6.2-fold on addition of 2.0% glycine ethyl ester hydrochloride (GEE) as compared with the production of a control culture. The addition of glycine, D-tryptophan, and DL-α-amino-n-butyric acid gave high released/total rations of enzyme production. On addition of inorganic phosphate to the media containing glycine, L-glutamic acid, and GEE, the enzyme production was repressed appreciably. The suitable carbon sources glycerol and glucose were usually required for the enzyme production even in the presence of glycine, L-glutamic acid, and GEE. The optimum pH was 10.5-11.2. The enzyme acted on various monophosphates, but did not hydrolyze 3', 5'-cAMP or bis (p-nitrophenyl) phosphate.
- 公益社団法人日本生物工学会の論文
- 1990-02-25