The Relaxation of Specificity of BanI Restriction Endonuclease from Bacillus aneurinolyticus IAM 1077

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概要

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• The restriction endonuclease BanI from Bacillus aneurinolyticus IAM 1077,which recognized 5'-GGPyPuCC-3' and cleaves between G and G within this sequence, has decreased substrate specificity at high nuclease concentrations. The relaxation of its specificity was enhanced during modified reactions : digestion of pBR322 DNA or lambda DNA in the presence of high glycerol and dimethyl-sulfoxide (DMSO) produced additional fragments in addition to the inherent fragments. Therefore, it is required to check the reaction conditions carefully for generation of inherent fragments.

• 社団法人日本生物工学会の論文
• 1990-02-01

著者

• Maekawa Yoshihiko

  Turuga Enzyme Plant Toyobo Co. Ltd.

• KAWAKAMI BUNSEI

  Turuga Enzyme Plant, Toyobo Co. Ltd.,

• Kawakami B

  Toyobo Co. Ltd. Fukui Jpn

関連論文

• The Relaxation of Specificity of BanI Restriction Endonuclease from Bacillus aneurinolyticus IAM 1077

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