Purification and Properties of an Alcohol Dehydrogenase (ADHc) of a Mutant Strain of Schwanniomyces castellii : SC-ADH-5P

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概要

• 論文の詳細を見る

• Alcohol dehydrogenase (alcohol NAD^+ oxidoreductase, EC 1.1.1.1) from Schwanniomyces castellii SCADH 5P strain has been purified 54-fold by affinity chromatography on Cibacron-blue. The enzyme is monomeric and has a molecular weight of 45000. The kinetic studies show that the mechanism of reaction is only compatible with an ordered sequential mechanism in which the cofactor is the first fixed substrate. The enzyme was found to oxidize primary alcohols and pliphatic aldehydes. The presence of an "α" double bond increases the enzyme activity both for alcohols and aldehydes.

• 社団法人日本生物工学会の論文
• 1989-12-25

著者

• GALZY Pierre

  Chaire de Genetique et Microbiologie, Ecole Nationale Superieure Agronomique

• Moulin G

  Ensa Montpellier Fra

• Moulin Guy

  Chaire De Genetique Et Microbiologie Ensa-inra

• MOUILLET-LOEVENBRUCK DOROTHEE

  Chaire de Genetique et Microbiologie , ENSA-INRA

• NICOLAS MICHEL

  Laboratoire de Biologie Cellulaire et Moleculaire, INSERM U65, USTL, place E. Bataillon

• Galzy Pierre

  Chaire De Genetique Et Microbiologie Ecole Nationale Superieure Agronomique

• Galzy Pierre

  Chaire De Genetique Et Microbiologie Ensa-inra

• Mouillet-loevenbruck Dorothee

  Chaire De Genetique Et Microbiologie Ensa-inra

• Nicolas Michel

  Laboratoire De Biologie Cellulaire Et Moleculaire Inserm U65 Ustl Place E. Bataillon

• GALZY PIERRE

  Chaire de Genetique et Microbiologie , ENSA-INRA

• MOULIN GUY

  Chaire de Genetique et Microbiologie , ENSA-INRA

• GALZY Pierre

  Chaire de Génétique et Microbiologie, INRA-ENSA

関連論文

• A Study of the Mechanism of the Reactions Catalyzed by the Amidase Brevibacterium sp. R312(Biological Chemistry)
• Purification and Properties of the Phytase from Schwanniomyces castellii
• Purification and Properties of an Alcohol Dehydrogenase (ADHc) of a Mutant Strain of Schwanniomyces castellii : SC-ADH-5P
• Purification and Characterization of an Adipamidase from a Mutant Strain of Brevibacterium sp. Involved in Dinitrile Degradation
• Benzyl Alcohol Oxidase and Laccase Synthesis in Botrytis cinerea
• A Very Stable β-Glucosidase from a Candida molischiana Mutant Strain : Enzymatic Properties, Sequencing, and Homology with Other Yeast β-Glucosidases
• Esterase Activities of Brevibacterium sp. R312 and Brevibacterium linens 62
• Study of a Diacetyl Reductase in a Polyploïd Brewing Strain of Saccharomyces carlsbergensis

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