Amylase Production in Submerged Culture Using Principal Component Analysis
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概要
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The aim of our work was to study the production of α-amylase and glucoamylase in submerged culture by a species of Aspergillus niger screened for its high amylase activity. Statistical analysis was not used to optimize fermentation conditions but it was employed in a new approach to describe the culture behaviour. Principal component analysis (PCA) was applied to describe the influence of three agitation speeds on the rate of amylases synthesis and on the evolution of other indirect growth factors. The results of this analysis showed that the oxygen transfer at various agitation speeds affected the levels of enzymes, their production rates and the CO_2 production rate (indirect measurements of growth rate). Maximal α-amylase synthesis was observed at the lower agitation rate whereas the glucoamylase synthesis was maximum at the intermediate agitation rate.
- 社団法人日本生物工学会の論文
- 1989-11-25
著者
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Rousset Sylvie
Station De Researches Sur La Viande Institut National De La Researche Agronomique Inra Theix
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SCHLICH PASCAL
Laboratoire de Recherches sur les Aromes, Institut National de la Recherche Agronomique (INRA)
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Schlich Pascal
Laboratoire De Recherches Sur Les Aromes Institut National De La Recherche Agronomique (inra)