High-Level Expression of Human Erythropoietin cDNA in Stably Transfected Namalwa Cells

概要

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We have optimized the expression of an introduced DNA coding for human erythropoietin (EPO) in stably transfected human B lymphoblastoid Namalwa cells. The results of transient expression of genomic and cDNA for EPO under the control of the SV40 early promoter-enhancer unit suggested that cDNA yields higher expression of EPO than genomic DNA. An intervening sequence was required for efficient expression, although even an intronless transcriptional unit could still express EPO. Stable Namalwa cells that secreted 1 to 2μg of EPO/10^6 cells/d were then established by transfection with DNA constructs consisting of the SV40 early promotor, EPO cDNA, and the splicing junction and polyadenylation site dericed from the SV40 T antigen gene or the rabbit β-globin gene. One of these transformant cell lines was easily adapted to grow in serum-free medium.
社団法人日本生物工学会の論文
1989-10-25