Optimization of Anthocyanin Yield in a Mutated Carrot Cell Line (Daucus carota) and Its Implications in Large Scale Production

概要

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Plant secondary metabolites have received much attention in recent years. Compared to many other plant secondary metabolites, anthocyanins are relatively inexpensive because many natural sources are avilable. Production of anthocyanin via cell culture technology is possible but its marketability depends on developing a cost-effective procedure. Since carbohydrates were the most expensive medium component in anthocyanin production, alternative sources of carbohydrates and mixtures of carbohydrates were studied for their effects on vell biomass invrease and product accumulation in a mutated carrot cell line. Galactose-glucose and galactose-sucrose combinations supported better growth and anthocyanin production than any single sugar. Inoculum densities produced marked effects on anthocyanin production. Based on these results, a cost-effective process strategy is discussed for the commercial scale production of anthocyanin from carrot cell cultures.
公益社団法人日本生物工学会の論文
1989-08-25