Construction of T-Tailed Vectors Derived from a pUC Plasmid : a Rapid System for Direct Cloning of Unmodified PCR Products
スポンサーリンク
概要
- 論文の詳細を見る
We consructed two new T-vectors called pUCTA119 and pUCTA18, derived from pUC18. The vectors were designed to produce single thymidine (T)-overhangs when digested with a restriction enzyme Eam1105I. The use of the vectors provides a very rapid system for direct TA cloning and subsequent sequencing of unmodified PCR products since Taq DNA polymerase preferentially adds an adenosine (A) residue to the 3' end of the products under standard PCR conditions.
- 1997-10-23
著者
-
HAYAMI Masanori
Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University
-
Ido Eiji
Department Of Pathology Kochi Medical School
-
Ido E
Kyoto Univ. Kyoto Jpn
-
Ido Eiji
Laboratory Of Pathogenic Virus Institute For Virus Research Kyoto University
-
Hayami Masanori
Laboratory Of Pathogenic Viruses Institute For Virus Research Kyoto University
関連論文
- Construction and In Vitro Properties of Chimeric Simian and Human Immunodeficiency Virus with the Human TNF-Alpha Gene
- Granular cell tumor expressing myogenic markers in the prostate
- Intrathymic Effect of Acute Pathogenic SHIV Infection on T-Lineage Cells in Newborn Macaques
- PHYLOGENETIC ANALYSIS OF HTLV-1 AND STLV : The Past Movement of HTLV-1-Carriers and the Origin of PTLV
- Prevalence and Phylogenetic Analysis of HTLV-I Isolates in Cameroon, Including Those of the Baka Pygmy
- Isolation of STLV-I from Orangutan, a Great Ape Species in Southeast Asia, and Its Relation to Other HTLV-Is/STLV-Is
- Adult T cell leukemia/lymphoma with massive involvement of cardiac muscle and valves
- Melanosis of the prostate: an immunohistochemical study
- Construction of T-Tailed Vectors Derived from a pUC Plasmid : a Rapid System for Direct Cloning of Unmodified PCR Products
- Infection of an HIV-1/SIVmac Chimeric Virus Having HIV-1 env to Macaque Monkeys via the Vaginal Cavity
- Immunohistochemical characterization of a case of duodenal gangliocytic paraganglioma
- Lung squamous cell carcinoma producing both parathyroid hormone-related peptide and granulocyte colony stimulating factor
- Mucinous cholangiocarcinoma featuring a unique microcystic appearance
- A New Approach to AIDS Research and Prevention:The Use of Gene-Mutated HIV-1/SIV Chimeric Viruses for Anti-HIV-1 Live-Attenuated Vaccines
- Infectivity and Immunogenicity of SIVmac/HIV-1 Chimeric Viruses (SHIVs) with Deletions in Two or Three Genes (vpr, nef and vpx)
- ETHNIC VARIATION OF HTLV IN THE WORLD
- Genetic origins of the Ainu inferred from combined DNA analyses of maternal and paternal lineages