Factors Affecting the Synthesis of the N-Terminal Methionine-Free Molecule of Recombinant Human Interleukin-2 by Escherichia coli

概要

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Escherichia coli harboring the gene encoding human interleukin-2 (IL-2) produces a mixture of two recombinant IL-2 species : one with the amino-terminal alanine (rIL-2) and the other having an additional methionine residue at the amino terminus (Met-rIL-2). Ways to increase the amount of rIL-2 and its proportion to the total IL-2 were tried. Among E. coli K-12 derivatives, N4830 was an effective producer of recombinant IL-2. The production of the mixture was greatly increased by optimizing the medium ingredients or culture conditions. However, the percentage of rIL-2 in the product decreased almost linearly with an increase of the total production of recombinant IL-2 and was less than 10% under optimal culture conditions. By adding 4.1×10<^-5> M manganese and 7.4×10^<-5>M ferric ions to the medium, we succeeded in raising the percentage of rIL-2 to 50% without any decrease of the total production.
社団法人日本生物工学会の論文
1988-04-25