Purification and Properties of a New Enzyme, Propioin Synthase in Baker's Yeast Which Forms Propioin from Propoinaldehyde
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概要
- 論文の詳細を見る
The new enzyme, propioin synthase, concerned with the formation of propioin from propionaldehyde was purified 270-fold from the crude enzyme in a yield of 28% by protamine sulfate precipitation, ammonium sulfate fractionation and G-200 gel chromatography using citrate-phosphate buffer (0.1 M Na_2HPO_4-0.02 M citric acid, pH 6.8,containing 0.33 mM MgSO_4,0.1 mM thiamine pyrophosphate, 2.5 mM MnSo_4 and 30 mM β-mercaptoethanol). The purified enzyme was homogeneous on disc gel electrophoresis. It was most active at pH 6.8-7.0 and 37℃, and stable at pH 7-8 and below 45℃. Its activity was enhanced by FeSO_4・7H_2O, MnSO_4,thiamine pyrophosphate, β-mercaptoethanol, MgSO_4,CaCO_3,and NaCl, and inhibited by AgNO_3,HgCl_2,CuSO_4,ZnSO_4,SnSl_2,NH_4Cl, (CH_3COO)_2Pb・3H_2O, iodoacetic acid, FeCl_3・6H_2O, and (NH_4)_2SO_4. Its molecular weight was 96,000 by sedimentation equilibrium, and 100,000 by Sephadex G-200 column chromatography.
- 公益社団法人日本生物工学会の論文
- 1988-02-25
著者
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Oshima T
Shoin Women's University Shoin Women's College
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MORIMOTO SHIGEMI
Shoin Women's University, Shoin Women's College
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OSHIMA TOMOKO
Shoin Women's University, Shoin Women's College
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Azuma Kazuko
Shoin Women's University Shoin Women's College
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SAKAMOTO MICHIKO
Shoin Women's University, Shoin Women's College
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Morimoto Shigemi
Shoin Women's University Shoin Women's College
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Morimoto Shigemi
Shoin Junior College Shoin Women's University
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Sakamoto Michiko
Shoin Women's University Shoin Women's College
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OSHIMA TOMOKO
Shoin Women's University, Shoin Women's College
関連論文
- Purification and Properties of Aldose Reductase from Pachysolen tannophilus
- Purification and Properties of D-Xylulose Reductase from Pachysolen tannophilus
- Purification and Properties of a New Enzyme, Propioin Synthase in Baker's Yeast Which Forms Propioin from Propoinaldehyde
- パン酵母によるプロピオンアルデヒドの代謝に関する研究〔英文〕