The Production of β-Glucosidase in Shake-Flasks by Aspergillus wentii

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概要

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• Studies in shake-flasks showed that Aspergillus wentii produces the maximum activity of β-glucosidase among the cultures tested. The activity against cellobiose was about 2-3 fold that against 4NPG. Aspergillus wentii produced a maximum activity of 16.5 U/ml in 14 days on malt extract. It also produced a comparable amount on other simple soluble sugars, which indicates that it is constitutive and does not require an inducer. Peptone was found to be the best nitrogen source for β-glucosidase production. Optimum C/N ratio was found to be 7.3. Phosphate, magnesium and trace metals did not play significant roles in the production of β-glucosidase when they were used with malt extract as a carbon source. An inoculum of 6% (v/v) of 20-h-old culture grown on malt extract produced the maximum β-glucosidase activity.

• 社団法人日本生物工学会の論文
• 1987-02-25

著者

• Ramachandran K

  Indian Institute Of Technology Delhi Hauz Khas

• SRIVASTAVA S.K.

  Indian Institute of Technology, Delhi, Hauz Khas

• GOPALKRISHNAN K.S.

  Indian Institute of Technology, Delhi, Hauz Khas

• RAMACHANDRAN K.B.

  Indian Institute of Technology, Delhi, Hauz Khas

• Srivastava S.k.

  Indian Institute Of Technology Delhi Hauz Khas

• Gopalkrishnan K.s.

  Indian Institute Of Technology Delhi Hauz Khas

関連論文

• The Production of β-Glucosidase in Shake-Flasks by Aspergillus wentii

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