Solid phase Fermentation of Fodder Beets for Ethanol Production : Effect of Grind Size

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Solid phase fermentation of pulped fodder beets was studied to see what effect beet particle size had on various fermentation parameters. All trials were run in 4-l stainless steel containers and hammermilled pulp was initially adjusted to pH 3.0 to control bacterial contaminants. The maximum yeast population that built up in the pulp was independent of the hammermill screen size (0.476-1.905 cm) and averaged 2.0-2.3×10^8 cells/ml. Pulp from finer screens (0.476-0.953 cm) took 19-22 h to reach a peak yeast population while pulp from coarser screens (1.270-1.905 cm) took a slightly longer 24-28 h. The time to reach maximum ethanol concentration was not affected by screen size and averaged 28-30 h. Ethanol yields dropped slightly from 85-87% of theoretical with the finest screens to 83-84% with the coarsest screens. The maximum ethanol concentration observed was 7.96% (v/v) and the average of all runs was 7.63% (v/v). Fermentation efficiency averaged 98-99% throughout. The lack of a response to grinding fodder beets with different screens was due to their wet fibrous nature which hindered free flow of pulp through the screens. Pulp was, instead, extruded through the screens, forming particles of generally similar size. Our results indicate that the primary consideration for grind size is energy consumption for grinding. Therefore, if a hammermill is used, a large screen (1.270-1.905 cm) which requires less energy should be employed so as to minimize energy consumption. This strategy does not result in longer fermentation times or reduced ethanol yields.
公益社団法人日本生物工学会の論文
1986-04-25

Solid phase Fermentation of Fodder Beets for Ethanol Production : Effect of Grind Size

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