Amylase from Lactobacillus cellobiosu Isolated from Vegetable Wastes

概要

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A rod shaped, non-motile, non-sporulating, Gram-positive bacterial strain, no.D-36,was isolated from vegetable wastes in the course of screening starch-hydrolysing microbes from municipal garbage. its growth and production of enzyme attained a maximum at pH 7.0 and temperature 37℃. it exhibited growth in MRS medium and utilized D-ribose without gas formation. Catalase activity, benzidine reaction, and production of indole and H_2S were negative. The enzyme production was enhanced when glycogen or starch, peptone, nicotinic acid, and Tween-80 were incorporated into the fermentation medium. Ca^<2+> had the most stimulating effect for growth as well as enzyme formation. Among the natural starchy wastes, rotten potato revealed maximum amylase synthesis. The strain was identified as Lactobacillus cellobiosus D-39,adding the new information of having α-amylase activity.
公益社団法人日本生物工学会の論文
1984-10-25