A Low-Cost Method for the Production of Extracellular Alkaline Proteinase using Tapioca Starch

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概要

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• Tapioca starch was liquefied using β-amylase of sweet potato by a simple procedure and this tapioca starch hydrolyzate was used as an inexpensive source of carbon for the production of extracellular alkaline proteinase. Of about 45 strains investigated for the production, Streptomyces moderatus NRRL 3150 was found to produce the maximum amount of the enzyme. The tapioca starch hydrolyzate was found to give more production than some of the conventionally used carbon sources such as glucose, maltose and soluble starch. The highest amount of extracellular proteinase was obtained on a medium containing 35 g tapioca starch hydrolyzate, 20 g soybean medl, 3 g CaCO_3 and 2 g NaCl per litre of water between pH 6.0 and 7.0. The production of the enzyme was carried out in a laboratory fermentor and the maximum proteolytic activity was observed after 120 h of cultivation.

• 社団法人日本生物工学会の論文
• 1983-10-25

著者

• Dhar S.c.

  Biochemistry Laboratory Central Leather Research Institute

• CHANDRASEKARAN S.

  Biochemistry Laboratory, Central Leather Research Institute

• Chandrasekaran S.

  Biochemistry Laboratory Central Leather Research Institute

関連論文

• A Low-Cost Method for the Production of Extracellular Alkaline Proteinase using Tapioca Starch

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