Cellulase Production by a Strain of Myrothecium sp.

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概要

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• A selected strain of Myrothecium sp. was grown on various cabon sources. Cellulose was found to be the highest inducer of cellulase. CMC resulted in a moderate yield. Cellobiose resulted in a low yield. Glucose, lactose, maltose and soluble starch resulted in negligible amounts. Sucrose, glycerol and salicin were extremely unsitable. Continuous addition of glucose or cellobiose during fermentation of cellulosic culture media reduced cellulase production, whereas addition of the entire amount of glucose or cellobiose at the beginning did not affect the enzyme production. The enzyme was precipitated from the culture filtrate with ammonium sulfate giving crude cellulase, 3854 units/g. The culture filtrate was concentrated to a one-tenth volume, 97 units/ml. The purified cellulase was prepared by dialysis, 6,700 units/g of enzyme precipitate.

• 1982-08-25

著者

• Kassim E.

  National Research Centre

関連論文

• Cellulase Production by a Strain of Myrothecium sp.

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