Continuous Biomass Production from Methanol by Methylomonas clara

概要

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By applying screening methods for C_1-utilizing microorganisms, a new Methylomonas species, Methylomonas clara, has been isolated from sludge. The organism was adjusted to technical scale by carrying out batch fermentations, scaling up from 30 to 3,000 l fermentors. Cultivation conditions were evaluated and optimized in these fermentations by applying the chemostat principle in bench-scale fermentors. In conjunction with this procedure a comparison of different fermentation reactors led to a theoretical understanding of the principles determing the biological process. This knowledge was used to develop and optimise a suitable reactor taking into account parameters such as cell productivity, yield, quality of product and energy consumption. Employing this technology, the strain Methylomonas clara was cultivated in a technical-scale reactor under aseptic and continuous conditions. For biomass recovery various concentration techniques were studied : chemical, electrical, thermal and surfactant flocculation methods, as well as centrifugation, filtration, and decantation techniques. An appropriate combination of these was chosen for the continuous concentration process and tested on a technical scale, the liquid phase being recycled to the fermentor. Finally, from several drying methods spray drying with granulation was employed to give a coarse, dry product, with high protein quality.
公益社団法人日本生物工学会の論文
1977-12-25