Growth Yields of Chlorella regularis in Dark-heterotrophic Continuous Cultures Using Acetate : Studies on Chlorella regularis, Heterotrophic Fast-Growing Strain (III)

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Acetate-limited chemostat and oxygen-limited turbidostat cultures (dark and aerobic) of Chlorella regularis S-50,using acetate as the sole carbon source, were established to study the growth characteristics of the alga under dark-heterotrophic conditions. Since the algal cell shows a poor tolerance to acetate, turbidostat cultures based upon pH-stat were performed under operating conditions almost identical to those of oxygen-limited chemostat cultures. In the oxygen-limited cultures, the sum of the amount of carbon in the cells grown and the carbon dioxide evolved was nearly equal to the quantity of carbon in the acetate consumed by the alga. Energetic analyses gave values of true growth yields, Y_G=25.6 (g cell/mole acetate), Y_<G0>=23.8 (g cell/mole O_2) and Y_<G'>=0.12 (g cell/kcal), and values of maintenance coefficients, m=0.2 (m mole acetate/g cell・hr), m_0=0.4 (m mole O_2/g cell・hr) and m'=0.042 (kcal/g cell・hr), respectively. It was confirmed that growth yields for acetate and oxygen in the oxygen-limited culture were apparently higher than those in the acetate-limited one.A cell productivity of 8.6 g cell/l・hr was obtained with a rate of oxygen consumption of 0.36 mole O_2/l・hr, when the oxygen-limited turbidostat culture was carried out by feeding fresh medium containing 140 ml acetic acid/l. The content of cellular protein and of chlorophyll was 40-65% and 1.0-2.5% per dry cell weight, respectively. These values tended to decrease with the increase of residual acetate in the culture broth.
公益社団法人日本生物工学会の論文
1977-08-25

Growth Yields of Chlorella regularis in Dark-heterotrophic Continuous Cultures Using Acetate : Studies on Chlorella regularis, Heterotrophic Fast-Growing Strain (III)

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