Monomethoxypolyethylene Glycol-Modified Cardiac Myosin Treatment Blocks the Active and Passive Induction of Experimental Autoimmune Myocarditis

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Background Injecting various protein antigens conjugated to monomethoxypolyethylene glycol (mPEG) results in antigen-specific tolerance to subsequent immunization. In the present study the ability of mPEG-modified cardiac myosin (CM) to block the development of experimental autoimmune myocarditis (EAM) induced by CM immunization or by the transfer of lymphocytes from CM-immunized donors was studied. Methods and Results A/J mice were injected with mPEG-CM before active or passive EAM induction. We examined the suppressive mechanism by the transfer of lymphocytes from mPEG-CM-treated mice into naive mice. To ascertain the cells responsible for suppressing EAM induction, in vivo or in vitro depletion of CD4^+ or CD8^+ T cells was performed. mPEG-CM administered before active or passive EAM induction markedly suppressed the incidence and severity of EAM and reduced CM-specific antibody responses. When lymphocytes from mPEG-CM treated mice were transferred into naive mice that were then immunized with CM, the suppressive effect was recapitulated. Conclusions mPEG-CM treatment blocked the active and passive induction of EAM.
社団法人日本循環器学会の論文
2004-01-20