NADPH-Dependent D-Aldose Reductases and Xylose Fermentation in Fusarium oxysporum (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

スポンサーリンク

概要

• 論文の詳細を見る

• Two aldose (xylose) reductases (ARI and ARII) from Fusarium oxysporum were purified and characterized. The native ARI was a monomer with M_r 41000, pI 5.2 and showed a 52-fold preference for NADPH over NADH, while ARII was homodimeric with a subunit of M_r 37000, pI 3.6 and a 60-fold preference for NADPH over NADH. In this study, the influence of aeration and the response to the addition of electron acceptors on xylose fermentation by F. oxysporum were also studied. The batch cultivation of F. oxysporum on xylose was performed under aerobic, anaerobic and oxygen-limited conditions in stirred tank reactors. Oxygen limitation had considerable influence on xylose metabolism. Under anaerobic conditions (O vvm), xylitol was the main product with a maximum yield of 0.34 mole of xylitol/mole of xylose while the maximum ethanol yield (1.02 moles of ethanol/mole of xylose) was obtained under aerobic conditions (0.3 vvm). When the artificial electron acceptor acetoin was added to an anaerobic batch fermentation of xylose by F. oxysporum, the ethanol yield increased while xylitol excretion was also decreased.

• 公益社団法人日本生物工学会の論文
• 2004-05-25

著者

• Christakopoulos Paul

  Biotechnology Laboratory Department Of Chemical Engineering National Technical University Of Athens

• Christakopoulos Paul

  Biotechnology Laboratory School Of Chemical Engineering National Technical University Of Athens

• PANAGIOTOU GIANNI

  Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens

• Panagiotou Gianni

  Biotechnology Laboratory School Of Chemical Engineering National Technical University Of Athens

関連論文

• A Novel Lipolytic Activity of Rhodotorula glutinis Cells : Production, Partial Characterization and Application in the Synthesis of Esters
• Mode of Action of a Minor Xylanase from Thermoascus aurantiacus on Polysaccharides and Model Substrates
• Enzymic Production of Aldopentauronic Acid and Use as a Bioregulator in Plant Airlift Bioreactors(PLANT BIOTECHNOLOGY)
• NADPH-Dependent D-Aldose Reductases and Xylose Fermentation in Fusarium oxysporum (MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

スポンサーリンク