Purification and Properties of a Low-Molecular-Weight Phytase from Cladosporium sp. FP-1 (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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A fungus producing high levels of phytase was isolated from air and identified as Cladosporium sp. The phytase production was stimulated by phytate in the medium used. The maximum production of phytase (108 U/ml) occurred in a medium containing 1.0 g of phytate per 100 ml. The phytase was purified to electrophoretic homogeneity by ion-exchange chromatography and gel filtration. Based on SDS-PAGE analysis, the molecular weight of the purified phytase was calculated to be approximately 32.6 kDa, and the narrow protein band indicated that this phytase is not glycosylated. The phytase has an optimum pH of 3.5, and an optimum temperature of 40℃. The phytase activity was stimulated by 2-mercaptoethanol and dithiothreitol, and inhibited by Ba^<2+>, Pb^<2+>, iodoacetate, p-chloromercuribenzoate and phenylmethylsulfonyl fluoride. The phytase displayed high affinity for phytate and the K_m was 15.2±3.1 μM. NMR analyses (1D and 2D) indicated that the end hydrolysis product of phytate was myo-inositol 1, 2, 5-triphosphate.
- 社団法人日本生物工学会の論文
- 2004-04-25
著者
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Kikuchi J
Graduate School Of Materials Science Nara Institute Of Science And Technology
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TIAN Wen-Jie
Graduate School of Materials Science, Nara Institute of Science and Technology
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FAN Sheng-Di
Department of Biological Engineering, Dalian Nationalities University, Economical and Technological
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KIKUCHI Jun-ichi
Graduate School of Materials Science, Nara Institute of Science and Technology
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QUAN CHUN-SHAN
Department of Bio-Engineering, Dalian Nationalities University, Economical and Technological Develop
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Fan Sheng-di
Department Of Biochemical Engineering Dalian Nationalities University
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Fan Sheng-di
Department Of Bio-engineering Dalian University For Nationalities Economical And Technological Devel
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Tian Wen-jie
Graduate School Of Materials Science Nara Institute Of Science And Technology
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Quan Chun-shan
Department Of Bio-engineering Dalian Nationalities University Economical And Technological Developme
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Kikuchi Jun-ichi
Graduate School Of Materials Science Nara Institute Of Science And Technology
関連論文
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