Selection of a Suitable Strain from Recombinant Escherichia coli Strains with the Same Genetic Structure Expressing Periplasmic hGM-CSF(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

概要

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The selection of a suitable strain among five recombinant Escherichia coli strains with the same genetic structure that expresses the human granulocyte macrophage colony stimulating factor (hGM-CSF) was carried out based on four criteria: growth rate, expression level, plasmid stability and feasibility for protein extraction. There was no significant difference in growth between the five strains, while a suitable expression level, a high plasmid stability and a good feasibility for protein extraction from periplasmSc space were observed for one of the recombinant strains. This strain expressed 27% hGM-CSF relative to total proteins and had 96% plasmid stability after 7-d subcultures on an antibiotic-free LB medium.
社団法人日本生物工学会の論文
2003-12-25