Inhibition of Proteases during Fermentation Improves Xylanase Production by Alkali Tolerant Aspergillus fumigatus AR1

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概要

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• An alkali-tolerant Aspergilus fumigatus AR1 produced a high level of xylanase (228 U/ml) at 42 h of submerged fermentation at pH 9.0. During later culture periods, xylanase activity declined considerably and reached 45 U/ml at 90 h. This trend is because of proteolytic degradation of xylanases since A. fumigatus co-produced multiple proteases with high activity (120-159 U/ml) during submerged fermentation. Xylanase and protease were found to be active over a wide range of pH from 5.5 to 9.0. In contrast, protease lost its activity completely in the presence of CuSO_4 (25 mM) while xylanase remained active. The selective inhibition of proteases by the addition of CuSO_4 to the culture medium prevented proteolytic degradation of xylanase and improved the production of xylanase by 3-fold

• 公益社団法人日本生物工学会の論文
• 2003-10-25

著者

• Gunasekaran P

  Madurai Kamaraj Univ. Madurai Ind

• ANTHONY THANGAMANI

  Dept. of Microbial Technology, School of Biological Sciences, Center for Excellence in Genomic Scien

• RAJ KRISFINAN

  Department of Chemical Engineering, Indian Institute of Technology

• RAJENDRAN AYYAPPAN

  Research Center in Botany, VHNSN College

• GUNASEKARAN PARAMASAMY

  Dept. of Microbial Technology, School of Biological Sciences, Center for Excellence in Genomic Scien

• Anthony Thangamani

  Dept. Of Microbial Technology School Of Biological Sciences Center For Excellence In Genomic Science

• Rajendran Ayyappan

  Research Center In Botany Vhnsn College

• Raj Krisfinan

  Department Of Chemical Engineering Indian Institute Of Technology

• Raj Krishnan

  Department of Chemical Engineering, Indian Institute of Technology

関連論文

• Inhibition of Proteases during Fermentation Improves Xylanase Production by Alkali Tolerant Aspergillus fumigatus AR1

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