Effect of Poly(β-Hydroxybutyrate) Accumulation on the Stability of a Recombinant Plasmid in Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

スポンサーリンク

概要

• 論文の詳細を見る

• Poly(β-hydroxybutyrate) (PHB) production using a recombinant Escherichia coli VG1 (pTU14) was carried out. PHB granules were gradually biosynthesized in recombinant cells as inclusion bodies and the morphology of cells became linearly inflated with PHB accumulation from 6 h to 36 h of culture. The stability of plasmid pTU14 was inevitably affected by in-cell PHB accumulation. During repeated subcultures under both PHB-accumulating and non-PHB-accumulating conditions, plasmid DNAs of pTU14 were extracted and analyzed after more than 60 generations. The results showed that plasmid pTU14 is structurally stable but segregationally unstable, and that the segregational instability worsened with increasing accumulation of PHB granules. This is due to not only the serious metabolic burden of plasmid replication and gene expression on the host cell, but also the volume effects of PHB granules on the natural random distribution of plasmids during the binary fission of cells.

• 社団法人日本生物工学会の論文
• 2003-08-25

著者

• Luo Hui

  Department Of Chemical Engineering Institute Of Biochemical Engineering Tsinghua University

• YU HUIMIN

  Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University

• SHI YUE

  Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University

• SUN XUDONG

  Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University

• SHEN ZHONGYAO

  Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University

• Shi Yue

  Department Of Chemical Engineering Institute Of Biochemical Engineering Tsinghua University

• Yu H

  Department Of Chemical Engineering Institute Of Biochemical Engineering Tsinghua University

• Sun X

  Department Of Chemical Engineering Institute Of Biochemical Engineering Tsinghua University

• Shen Z

  Department Of Chemical Engineering Institute Of Biochemical Engineering Tsinghua University

• Shen Zhongyao

  Department Of Chemical Engineering Biochemical Engineering Institute Tsinghua University

• LUO Hui

  Department of Biological Science and Technology, University of Science and Technology Beijing

• YU Huimin

  Department of Cardiology, Guangdong Provincial People's Hospital and Guangdong Cardiovascular Institute

関連論文

• Effect of Poly(β-Hydroxybutyrate) Accumulation on the Stability of a Recombinant Plasmid in Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• The role of nuclear factor-κB in the effect of angiotensin II in the paraventricular nucleus in protecting the gastric mucosa from ischemia-reperfusion injury in rats
• T1198C Polymorphism of the Angiotensinogen Gene and Antihypertensive Response to Angiotensin-Converting Enzyme Inhibitors
• Possibility to Improve the Cyanobacterial Poly-β-Hydroxybutyrate Biosynthesis Level
• Overexpression of synthesized cephalosporin C acylase containing mutations in the substrate transport tunnel(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Double knockout of β-lactamase and cephalosporin acetyl esterase genes from Escherichia coli reduces cephalosporin C decomposition(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Double knockout of β-lactamase and cephalosporin acetyl esterase genes from Escherichia coli reduces cephalosporin C decomposition

スポンサーリンク