Novel Gene Assay by Probe-Regulated Simultaneous Separation Using Capillary Electrophoresis (CE-PRESS) (BIOCHEMICAL ENGINEERING)
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概要
- 論文の詳細を見る
A novel methodology, a probe-regulated simultaneous separation using capillary electrophoresis (CE-PRESS), was developed for simultaneous assay of multiple genes. The single-stranded (ss) DNA-polymer conjugate-probes were placed in a capillary, and then a mixture of target ssDNAs was injected. These ssDNAs were hybridized with corresponding complementary ssDNA-polymer conjugate-probes after charging the capillary. Two resulting double-stranded (ds) complexes of ssDNAs and ssDNA-polymer conjugate-probes were detected at different migration times. We found that the electrophoretic mobilities of two ssDNA-poly (acrylamide) conjugate-probes [-(5'-GCCACCAGC-3')_m-AAm_n-and -(5'-ACCTTCACT-3')_p-AAm_q- ; AAm, acrylamide] obtained by copolymerizing 5'-methacryloyl-modified ssDNA and AAm were different, depending on their molar fraction of ssDNA, although the ssDNAs chain lengths were the same. Two ssDNAs (5'-GCTGGTGGC-3' and 5'-AGTGAAGGT-3') having the same chain length were successfully separated with our novel system, although the separation of these ssDNAs is impossible in conventional capillary electrophoresis systems.
- 社団法人日本生物工学会の論文
- 2003-07-25
著者
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Maeda Mizuo
Bioengineering Laboratory Riken
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Maeda Mizuo
Bioengineering Laboratory Riken (the Institute Of Physical And Chemical Research)
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Maeda Mizuo
Bioengineering Lab. Riken
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MURAKAMI YOSHIHIKO
BIoengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research)
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Murakami Yoshihiko
Bioengineering Laboratory Riken (the Institute Of Physical And Chemical Research)
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