The Microbial Community in a 2,4-Dinitrophenol-Digesting Reactor as Revealed by 16S Rdna Gene Analysis (ENVIRONMENTAL BIOTECHNOLOGY)

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概要

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• The microbial community of a 2,4-dinitrophenol-digesting reactor was investigated using different molecular biological techniques based on 16S rDNA gene se1uences. A PCR-denaturing gradient gel electrophoresis (DGGE) analysis of the bacterial community in the reactor showed that one strong and five minor bands were observed in the DGGE profile. The results of excising and sequencing DGGE bands suggested that members of Rhodococcus, Nocardioides, and Nitrospira species were present in the reactor. Partia sequencing of cloned 16S rDNAs revealed diversity among the six main divisions - the α, δ subclasses of Proteobacteria, Nitrospira, Cytophaga / Flexibacter / Bacteroides, Verrucomicrobia, and Actinobacteria - in the reactor. Two cloned sequence types were not closely affiliated with any described bacterial divisions. The isolatation and phylogenetic analysis of 2,4-DNP-degrading bacteria from the reactor revealed that isolated strains were classified into two types of bacteria having different 16S rDNA sequences. One of these strain types was identified as a relative of Rhodococcus koreensis, and the other was identified as a relative of Nocardioides simplex FJ21-A.

• 社団法人日本生物工学会の論文
• 2003-07-25

著者

• Lee T

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• Kimura N

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• Kimura Nobutada

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• SHINOZAKI YUKIKO

  Institute of Applied Biochemistry, University of Tsukuba

• LEE TAE

  Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science

• YONEZAWA YOSHITAKA

  Research Center for Chemical Risk Management, National Institute of Advanced Industrial Science and

• Shinozaki Y

  Institute Of Applied Biochemistry University Of Tsukuba

• Shinozaki Yukiko

  Institute Of Applied Biochemistry University Of Tsukuba

• Yonezawa Y

  Research Center For Chemical Risk Management National Institute Of Advanced Industrial Science And T

関連論文

• The Microbial Community in a 2,4-Dinitrophenol-Digesting Reactor as Revealed by 16S Rdna Gene Analysis (ENVIRONMENTAL BIOTECHNOLOGY)
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• Difference in Degrading p-Nitrophenol between Indigenous Bacteria in a Reactor
• Metagenomics : Access to Unculturable Microbes in the Environment

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