Monitoring Genetic Stability in Quercus serrata Thunb. Somatic Embryogenesis Using RAPD Markers
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概要
- 論文の詳細を見る
Genetic stability of propagules regenerated via somatic embryogenesis is of paramount importance for its application to clonal forestry. Random amplified polymorphic DNA (RAPD) markers were used to determine the genetic stability in somatic embryogenesis of Quercus serrata Thunb. (Japanese white oak). Forty samples from an embryogenic line, consisting of regenerated plantlets, somatic embryos, and embryogenic calli, were examined using 54 decanucleotide primers. A total of 6520 clear reproducible bands obtained from these studies exhibited no aberration in RAPD banding pattern among the tested samples. Our results show that somaclonal variation is absent in our plant propagation system. The genetic stability is discussed in terms of the origin of somatic embryos.
- 一般社団法人日本森林学会の論文
- 1999-05-16
著者
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ISHII Katsuaki
Forestry and Forest Products Research Institute
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Thakur Ramesh
Forestry and Forest Products Research Institute
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Goto Susumu
Fukuoka Prefecture Forest Research and Extension Center
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Jain S.
University of Helsinki
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ISHII Katsuaki
Forest Bio-Research Center, Forestry and Forest Product Research Institute
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