Characterization and Identification of Strobilomyces confusus Ectomycorrhizas on Momi Fir by RFLP Analysis of the PCR-Amplified ITS Region of the rDNA

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We identified ectomycorrhizal (ECM) roots formed by Strobitomyes confus associated with Abies firma based on molecular analyses combined with ECM descriptions and examined the correspondence between above-ground S. confusus fruiting bodies and ECM roots below ground. Both mature fruiting bodies of S. confusus and soil samples (1,OOO cm^3 in volume) from directly below the fruiting body were collected from three different spots in a 10×30-m plot in central Japan, in which A. firma was the dominant species. Total of 6,156 root tips was collected from the area directly under the fruiting bodies of S. confusus were classified representing 16 morphotypes. Among them, type 16 was identical in both the length of the internal transcribed spacer region and the restriction fragment length polymorphism profiles of Alu I or Hinf I digests. to those of the fruiting body of S. conftisus. Although a specific ECM association between S. confidsus and A. firma was confirmed by using molecular tools, the abundance of the ECM roots was consistently low in every soil sample tested. These results suggest that even directly beneath S. confusus fruiting bodies, the occurrence of this species above-ground does not accurately reflect its relative abundance below ground, as evaluated in terms of the number of ECM roots.
一般社団法人日本森林学会の論文
1999-05-16