Genotype Differencies in Direct Plant Regeneration from Stem Explants of Populus tremula in Turkey

概要

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Plant regeneration from 9 clones of Populus tremula representing diverse genotypes originated from Turkey, has been studied in different media, such as Murashige and Skoog's Medium (MS), Aspen Culture Medium (ACM) and Woody Plant Medium (WPM) with appropriate supplements of growth regulators. Regeneration efficiency on WPM containing 1 mg/l zeatin using stem explants in vitro, was higher than on MS and ACM. In this system, the time required for shoot regeneration was 2 weeks, which was shorter by two weeks than that of previous reports. A strong genotype dependence was observed among the tested nine clones. The regeneration system, we describe here, did not involve the callus phase and thus was less prone to somaclonal variation. When adventitious microshoots were cultured on WPM supplemented with 0.5 mg/l or 2 mg/l indole-3-butyric acid (IBA), root formation was obtained after 1 week of culture. Adaptation of regenerated plantlets in vivo was 100%. This system for micropropagation of Populus tremula is expected to be suitable for studies on transformation of genes involved in lignin biosynthesis via modification of lignin content.
日本森林学会の論文
1998-05-16