実験的腎性高血圧およびその腎静脈門脈系吻合犬における^131 I-AnogiotensinIIの代謝

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Purpose Renin-Angiotensin system is thought to play the most important role as the pathogenesis of human and experimental renal hypertension. The following studies were performed to clearify some factors of chronic renal hypertension and furthermore the effects of reno-portal venous anastomosis which was shown to be effective to reduce the renal hypertension. Experimental Method Adult mongrel dogs weighing 11 to 12kg were used in this study and were divided into following three groups ; Group I...Dogs with one side kidney (left) was removed and was normotensive (used as control) Group II...Dogs with left kidney removed and were made hypertensive for more than one month by stenosis of right renal artery by mean of metal clipping. Group III...Dogs were made hypertensive as described in Group II and then right renal vein was anastomosed end to side to portal vein. In these three groups of dogs, Angiotensin II, in the dose of 2.5μg/min./kg was infused intravenously for 30 minutes and the changes of B.P. were measured 2 to 5 minutes interval before, during and after the infusion. In Group I and II the urine was collected 20 minutes after the end of Angiotensin II i. v. infusion and Rf values were measured by paper chromatography using the mixture of n-butanol, acetic acid and water solution in the ratio of 4: 1:5 (v/v) as medium. Then, ^<131>I-Angiotensin II in the dose of 2.5g/min./kg was infused intravenously for 20 minutes after dogs were given Lugol solution. Thenafter, radioactive counts were measured by well-type scintillation counter in 1ml of the arterial blood and the urine obtained soon after the end of infusion and in 1gm Speimens of the brain (brain stem), heart, lung, liver, spleen, pancreas, kidney and calf muscle obtained immediately after dogs were sacrificed and autoradiography was also studied in the same materials as described above except urine, after these samples were homogenized with perchloric acid and centrifuged, separated and supernatant was neutralized by KOH and filtered.
社団法人日本循環器学会の論文
1966-12-20

実験的腎性高血圧およびその腎静脈門脈系吻合犬における^131 I-AnogiotensinIIの代謝

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