健常および動脈硬化症における血漿リポ蛋白構成脂質の脂酸構成に関する研究 : I.健常および動脈硬化症例におけるリポ蛋白脂酸構成について

スポンサーリンク

概要

論文の詳細を見る
Role of hyperlipemia to the development of arteriosclerosis is commonly recognized from experimental, clinical, etiological and nutritional aspects. Furthermore, by the application of gas chromatography to the fatty acid analysis, abnormality of plasma fatty acid composition has been reported in arteriosclerotic patients and confirmed. The plasma fatty acids are composed of esterified fatty acids which are contained lipoproteins, and albumin bound free fatty acids. And there exists such differences in metabolic pathway between these fatty acids that it seems very important to consider the disorder of fatty acid compositions in arterio-sclerosis from the aspect of plasma lipoproteins. In this study, the fatty acid compositions of ultracentrifugally separated various lipoproteins and albumin bound free fatty acid were determined in the normal and arteriosclerotic subjects and compared between both subjects. Furthermore transport of ingested linoleic acid was studied in the both groups. Method s Subjects and samples: 5 cases of male patients with coronary heart disease and 5 cases of matched control were utilized as subjects. Blood samples were obtained at the overnight fasting state. Thirty gr. of linoleic acids were administrated orally to the each 1 case of both groups of subjects. Then, blood samples were drawn 4 hours after administration. Biochemical procedures: 1) Sf20-10^5, SfO-20, high density lipoproteins and albumin fraction were fractionated by ultracentrifugation. 2) Various lipoprotein lipids and F. F. A. were separated by the technic of thin layer chromatography. 3) Fatty acid compositions were determined by the technic of gas chromatography. Results 1) Fatty acid compositions of same kind of lipid in various lipoprotein fractions were al-most consistent in the both subjects. 2) In the arteriosclerotic subjects, increase of palmitic and oleic acid percentages and de-crease of linoleic acid percentage were observed in the cholesterol ester and phospholipid fatty acid compositions. However, no changes were observed in the triglyceride fatty acid composition. Changes in fatty acid composition of F.F.A. were almost consistent with those in cholesterol ester and phospholipid. 3) 4 hours after the linoleic acid administration, increase of linoleic acid was observed in the triglyceride of all lipoprotein fractions and extent of increase was most significant in the Sf20-10^5 lipoprotein triglyceride. Futhermore, degree of increasing extents of linoleic acid were almost consistent between normal and arteriosclerotic subjects. In the F.F.A. increase of linoleic acid was also observed .
社団法人日本循環器学会の論文
1966-12-20

著者

関連論文

スポンサーリンク