心筋硬塞症のリウマチ血清反応に関する研究
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概要
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Recently there have been some trials on immunological studies on cardiovascular diseases. In myocardial infarction, a few studies have been conducted. The author directed his attention toward the attitude of serological reaction in rheumatoid arthritis as a part of the immunological examination in myocardial infarction. This paper presents the frequency of the positivity of the R. A. test, the relation between clinical features and the positive findings of R. A. test, and the mechanism of appearance of the positive factor of R. A. test in myocardial infarction. Materials and Methods Clinical materials totaled 88, consisting of 40 patients with myocardial infarction, 11 with angina pectoris, 16 with rheumatoid arthritis and 21 healthy persons as control. The materials used were sera obtained from each subject in this study and prepared sterilely. 1) R. A. Test : The reagents supplied by the Hyland Lab., U. S. A., were used for the R. A. test. In this test the quick slide method designed by SlNGER and PLOTZ and the dilution method were used. 2) Waaler-Rose Reaction modified by HELLER : Agglutination at a dilution of 1 : 64 or greater was recorded as 'positive'. 3) FII Test modified by KUMAGAI : O-group human erythrocyte was used instead of sheep erythrocyte, and the buffer to which was added human albumin at the rate of 0.5% was used. Other technique was according to the original method. Agglutination at a dilution of 1 : 200 or greater was recorded as ' positive '. 4) Gel Filtration : Serum protein was fractionated by gel filtration in Sephadex G-200. R. A. test was performed on each of these fractions. 5) Immunoelectrophoresis : This procedure was performed according to the method described by MIGITA. 6) Ultracentrifugation : A Spinco Model E ultracentrifuge was used to obtain the distribution of the protein.
- 社団法人日本循環器学会の論文
- 1965-10-20