実験的粥状硬化症における大動脈組織蛋白質の電気泳動的研究
スポンサーリンク
概要
- 論文の詳細を見る
Many studies have recentry been carried out on changes in the chemical structure of arterial tissues. However, almost all of these studies hitherto concerned themselves with lipids, whereas but few treated the aspect of protein, the presence of which is now known to be much more important than that of lipids for the arteriosclerotic changes in the arterial tissues.The present author therefore aims at investigating the changes in the water-soluble proteins of aortic tissues with special reference to the effect of the diet protein upon them. Methods : A lanolin : cotton seed oil mixture was administered to rabbits fed 250 cal. daily each, on different diets viz. the standard, high-protein and low-protein diets, of which the protein content being 11.2gm., 21.9gm. and 3.0gm., respectively.In order to acquire a sufficient concentration of protein solution neccessary for electrophoresis, every 5 rabbits fed the same diet were sacrificed to take out the thoracic aorta for preparing one sample of aortic tissue material. The aortic tissues were homogenized with a waring blender, after their adventitiae were removed. They were homogized again following the freezing and thawing treatment, at a constant speed of 14,000 r.p.m. for 30 min. till the supernatant became clear, by means of refrigerated centrifuge.The final supernatant was then dialyzed for 18-24 hrs. after 0.144 M. veronal buffer was added to adjust its pH to 8.45 (μ=0.144), and the electrophoresis was performed. The relative percentage composition was calculated by the gravity method using ascending pattern and mobility, by the delta peak. The aortic arch was used as the human aorta meterial and this was then treated with the same procedures as the rabbit aorta material. For the electrophoresis of rabbit serum protein, the same buffer was used and the calculations were done by using the descending patterns. Results : The normal electrophoretic pattern of the water-soluble protein of rabbit aortic tissues consisted of 4 peaks, which were nominated as fractions a, b, c and d, according to their mobilities in the decreasing order.The relative percentage composition ratios were a, 6.1±2.35%, b, 26.0±3.09%, c, 16.3±4.07%, d, 51.6±4.45% and the mobilities (10^<-5>cm^2/volt.sec.) were a, 12.63±2.869,b, 9.11±1.895,c, 6.15±1.497 and d, 3.53±0.944.Following the lipid administration to rabbits fed the standard diet, an increase in the relative percentage composition of c, and decrease in d were noted, when the atheromatous plaques made its appearance. These changes became more apparent in the phase of high degree atheroma formation, when the fraction c was increased up to 2.4 times, while d decreased down to 1/2 of the control.The high-protein diet fed and lipid-administered group showed similar changes in the fractions of intraaortic water-soluble protein, which, however, were relatively milder electrophoretically in comparison with the control group, although no significant difference was noticed in the atheromatous lesions between the two groups.On the other hand, the low-protein diet fed and lipid administered rabbits showed, early in the pre-hyperlipemic phase, the similar changes in the electrophoretic pattern, that were as marked as those observed after the development of macroscopic atheroma in the control.Following the lipid administration, albumin decreased and β-globulin increased as blood cholesterol as elevated in the rabbit sera of either of the three groups ; among them, fluctuations were the least remarkable in the high protein group.The electrophoretic pattern of the water-soluble protein of human aorta consisted of 6 peaks, which were nominated, according to their mobilities as fractions I, II, III, IV, V, and VI. The aorta tissue with atheromatous changes showed macroscopic increase in I, II, V, and decrease in III as compared with normal adult aortae, with no lesions of atherosclerosis.Summary : (1) The water-soluble protein of rabbit aortic tissues is
- 社団法人日本循環器学会の論文
- 1960-01-20