The Interaction of Acetylcholine Receptors in Porcine Atrial Membranes with Three Kinds of G Proteins : 53th Annual Scientific Session oh the Japanese Circulation Society
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概要
- 論文の詳細を見る
We developed a simple procedure to detect the interaction of muscarinic receptors in atrial membranes with exogenous GTP-binding proteins (G proteins). The procedure consists of mixing atrial membranes with G proteins in the presence of sodium cholate, diluting the mixture with a salt buffer and then measuring the ligand binding activity. The displacement by carbachol of [^3H] QNB binding to muscarinic receptors in the atrial membranes was not affected by guanine nucleotides when the membranes had been treated at 60℃ for 30 min or with N-ethylmeleimide (NEM) and became affected by them after mixing the heat- or NEM-treated membranes with G proteins. The displacement curves in the presence of GTP were essentially the same irrespective of the presence or absence of G proteins. Those in the absence of GTP shifted to a lower concentration of, carbachol with addition of a higher concentration of G proteins, indicating an increase in GTP-sensitive high affinity agonist binding sites. The highest affinity for carbachol was detected with membranes treated with NEM and then mixed with G proteins. The GTP-sensitive high affinity agonist binding could be detected with any one of three kinds of G proteins (Gi, Go, Gn) which were purified from porcine cerebrum, indicating that the muscarinic receptor m2 subtype may interact with and possibly activate these three kinds of G proteins.
- 社団法人日本循環器学会の論文
- 1990-09-20
著者
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Haga Tatsuya
Department Of Neurochemistry Faculty Of Medicine University Of Tokyo
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Haga Tatsuya
Department Of Biochemistry Institute Of Brain Research Faculty Of Medicine University Of Tokyo
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Haga Tatsuya
Department Of Biochemistry Institute For Brain Research Faculty Of Medicine The University Of Tokyo
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Haga Kazuko
Department Of Biochemistry Institute Of Brain Research Faculty Of Medicine University Of Tokyo
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IKEGAYA TAKAYOSI
Third Department of Internal Medicine, Hamamatsu University School of Medicine
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Ikegaya Takayosi
Third Department Of Internal Medicine Hamamatsu University School Of Medicine
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- The Interaction of Acetylcholine Receptors in Porcine Atrial Membranes with Three Kinds of G Proteins : 53th Annual Scientific Session oh the Japanese Circulation Society
- Effects of Toluene Exposure on Signal Transduction : Toluene Reduced the Signaling via Stimulation of Human Muscarinic Acetylcholine Receptor m2 Subtypes in CHO Cells
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- Purification and Funcional Reconstitution with GTP-Binding Regulatory Proteins of Hexahistidine-Tagged Muscarinic Acetylcholine Receptors(m2 Subtype)
- The Receptor-G.ALPHA. Fusion Protein as a Tool for Ligand Screening: a Model Study Using a Nociceptin Receptor-G.ALPHA.i2 Fusion Protein
- Effects of Partial Agonists and Mg2+ Ions on the Interaction of M2 Muscarinic Acetylcholine Receptor and G Protein Gαi1 Subunit in the M2-Gαi1 Fusion Protein