血管障害に占める腎皮質中の血管透過性因子の役割について

スポンサーリンク

概要

• 論文の詳細を見る

• The decisive role played by hypertension in the production of vascular lesions is well known. There is much argument as to whether endogenous pressor substances are able to produce vascular lesions. In our laboratory it has been clarified that a vascular permeability factor besides pressor factors is present in extracts of kidney cortex and might play an important role in the development of experimental cerebral hemorrhages in hypertensive rabbits caused by GOLD-BLATT technique. Thus the present studies were undertaken to investigate the biological and chemical properties of the vascular permeability factor obtained from human kidney cortex and its role in the pathogenesis of human vascular lesions, especially of cerebral hemorrhages. Materials and methods 1. Isolation of CPF in kidny tissue. Human kidneys obtained at autopsies were extensively perfused through a polyethylene tube inserted in the renal artery. The perfusion fluid was 10: 1 of saline added heparin. After the last traces of blood had been washed out medullar layer was removed and cortical layer cutted into small pieces which were washed with cold saline to eliminate the haemoglobin. The tissue was weighed and suspended in 2 vol. of the distilled water and then homogenized. The homogenates were kept over night in a cold room, and then centrifuged at 40000 × g for 30 minutes and the supernatant recentrifuged at 100000 g for 60 minutes. Sephadex G 100 and 1/50 M tris buffer were used for the gel filtration. 2. Preparation of antisera. The microsomal fraction isolated from human kidney cortex was treated with 0.5 per cent deoxycholate and was centrifuged at 105000 X g for 90 minutes. The supernatant thus obtained was separated into three sub-fractions by means of the fractional precipitation with ammonium sulphate. Sub-fraction 45 was dissolved with water and dialyzed against it. The antisere were obtained in rabbit using adjuvant technique.

• 社団法人日本循環器学会の論文
• 1967-09-15

著者

• 永井 忠之

  農協高岡病院

• 永井 忠之

  金沢大学医学部第二内科

関連論文

• 動脈硬化の成り立ちに関する研究 : 糖代謝と動脈硬化症について
• 50)動脈硬化症に関する研究 実験的高血圧に随半した動脈硬化症について(第29回日本循環器学会関東・甲信越・北陸地方会総会)
• 蔗糖摂取と血管障害
• 本態性高脂血症に関する研究 : 日本循環器学会第25回東海・第1回北陸合同地方会総会
• 脳卒中の成因に関する研究 : 腎皮質血管透過性亢進物質を中心に
• 血管損傷因子に関する研究 : 尿揮発成分の血管損傷作用について
• 14)特発性肥大性大動脈弁下狭窄症の治療経験 : 第21回日本循環器学会北陸地方会
• 血管障害に占める腎皮質中の血管透過性因子の役割について
• 49)粥状動脈硬化治療薬剤に関する研究 : CPIB誘導体について : 第34回日本循環器学会関東甲信越地方学会総会
• 168)動脈硬化治療薬剤に関する研究 : 講演会一般演題
• 62)腎虚血性変化の診断に関する研究 : 講演会一般演題
• 47)高血圧症のレノグラム, 腎シンチグラムに関する研究(第1報)(第30回日本循環器学会関東甲信越北陸地方会総会) : 実験的高血圧症における考察

もっと見る 閉じる

スポンサーリンク