腎皮質の血管損傷因子に関する実験病理学的研究

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Although there are numerous publications now in the literature on the pathogenesis of experimental hypertension, the mode of the development of cerebral hemorrhage has attracted little attention. In our laboratory a vascular permeability factor was demonstrated in the kidney cortex and we have reached to the working hypothesis that this factor might play an important role in the development of cerebral hemorrhages. It is the purpose of this report to clarify the property of the vascular permeability factor and its role in the development of vascular lesions in experimental animals. Materials and Methods 1. Production of experimental hypertension. Male rabbits weighing from 2.0 to 3.4 Kg were used in all experiments. A silver clip of 0.65 mm in diameter was applied to the right renal artery and after one week a 1.1 mm silver clip was applied to the left renal artery. They were divided into two groups. One group was given 10 per cent dehydrated lanolin (GB+L group), and the other was the control group (GB group). Blood pressures were measured in the central artery of the ear. 2. Preparation of vascular permeability factor from kidney cortex. After rabbit kidneys were perfused with physiological saline and heparin solution mixture, the cortex was precisely separated from the medulla and cut into small pieces about 2 to 3 mm in thickness. Hemoglobin was completely washed out with ice-cold physiological saline and the extract was made up by homogenization with twice the volume of distilled water. The homogenate was left overnight at 4'C for extraction, centrifuged at 40,000 G for 30 minutes and then the supernatant was re-centrifuged at 100,000 G for 60 minutes. The vascular permeability factor was further purified by chromatography on Sephadex G-100 column.
社団法人日本循環器学会の論文
1967-11-15

腎皮質の血管損傷因子に関する実験病理学的研究

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