Myocardial Energetics and Cardiac Function of Preserved Rat Heart : CLINICAL EVALUATION OF CARDIAC PERFORMANCE

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We studied mitochondrial function in relation to ATP production and its relationship with myocardial oxygen consumption (VO_2) and total mechanical energy using isolated rat hearts after 8, 12, and 24 h of hypothermic preservation. In isovolumic contraction, ventricular contractility and total mechanical energy were respectively assessed by the end-systolic elastance (Ees) and pressure-volume area (PVA). Ees significantly decreased after hypothermic ischemia, although the difference was not significant between 8 and 12 h. In contrast, VO_2 measured at each left ventricular volume increased after hypothermic ischemia. PVA and VO_2 Were found to stay in linear correlation after prolonged hypothermic ischemia, although VO_2 at null PVA and VO_2 to PVA ratios significantly increased after hypothermic ischemia, especially after 12-h ischemia. Mitochondrial oxidative phosphorylation significantly decreased after hypothermic ischemia for longer than 12 h. These results indicate that mitochondrial oxidative phosphorylation was impaired due to long time hypothermic ischemia especially after 12-h ischemia. We conclude that energy uncoupling between VO_2 and PVA in hypothermically preserved heart is attributable to disturbed mitochondrial oxidative phosphorylation and that 8 h is a critical point for efficient conversion of energy from VO_2 into PVA in rat heart.
社団法人日本循環器学会の論文
1992-07-20