Comparison of Endothelin Binding Sites in Cultured Aortic Smooth Muscle Cells From Spontaneously Hypertensive and Normotensive Rats

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Endothelin-1 (ET-1), which is secreted from vascular endothelial cells, is not only a potent vasoconstrictor but also a vascular smooth muscle cell growth factor. The direct effect of ET-1 on vascular smooth muscle cells, mediated via its specific receptor may therefore play an important role in hypertension and atherosclerosis. Our previous studies indicated that ET-1 secretion from cultured aortic endothelial cells from spontaneously hypertensive rats (SHRs) at the prehypertensive stage (4 weeks old) was not significantly different from that of cells from age-matched Wistar-Kyoto (WKY) rats. In this study, the binding of ET-1 to cultured aortic smooth muscle cells from SHRs and WKY rats was studied. Using tissue explant techniques, rat aortic smooth muscle cells from SHRs and age-matched WKY rats of different ages (4 and 24 weeks old) were successfully cultured in vitro. The maximum binding capacity (B_<max>) and binding affinity (K_d) of ET-1 to cultured aortic smooth muscle cells were evaluated by a receptor-binding assay. The data revealed that the affinity of ET-1 binding to smooth muscle cells was similar in all 4 groups of experimental rats. However, the B_<max> of cultured smooth muscle cells from 24-week-old SHRs was 2.5 times higher than of smooth muscle cells from age-matched WKY rats (8.64±0.72 vs 3.69±0.10 fmol/10^6 cells) and 1.5 times higher than in aortic smooth muscle cells from 4-week-old SHRs (8.64±0.72 vs 5.36±0.36 fmol/10^6 cells). These results suggest that hypertension in SHRs may be related to a high density of ET-1 receptors on arterial smooth muscle cells.
社団法人日本循環器学会の論文
1997-01-20

Comparison of Endothelin Binding Sites in Cultured Aortic Smooth Muscle Cells From Spontaneously Hypertensive and Normotensive Rats

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