Age-associated Decrease of OxidativeRepair Enzymes,Human 8-Oxoguanine DNA Glycosylases (hOgg1), in Human Aging

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概要

• 論文の詳細を見る

• 8-Oxoguanine has been shown to be a dominant cause of oxidative DNA damage by oxygen free radicals in eukaryotic cells. The 8-oxoguanine repair-specific enzyme 8-oxoguanine-DNA glycosylase (hOgg1) was recently cloned and was observed to conduct mainly short-patch base-excision repair. It has also been suggested that reactive oxygen species play an important role in the cellular aging process. We explored the association between the hOgg1 enzyme activity in somatic cells of human subjects of various ages and the role of hOgg1^<326> genetic polymorphism. An 8-oxoguanine-containing 28 mer oligonucleotide was end-labeled with γ-^<32>P ATP and incubated with protein extracts from peripheral blood lymphocytes (PBL) from 78 healthy individuals ranging in age from newborn to 91 years old. The hOgg1 repair activity toward the radiolabelled 8-oxoguanine-containing DNA was determined, and the results indicated a significant age-dependent decrease in the hOgg1 activity in their lymphocytes. Significantly reduced activity was also shown in those with Cysteine/ Cysteine genotypes. The genders of the subjects were not shown to be associated. These results provide an important observation regarding the cellular hOgg1 activity in somatic cells during the normal human aging processes.

• 日本放射線影響学会の論文

著者

• CHANG Wushou

  Institute of Public Health, National Yang Ming University.

• Wei Yau-huei

  Institute Of Biochemistry National Yangming University

• Chang Wushou

  Institute Of Environmental Health Sciences National Yangming University:national Miaoli General Hosp

• Chen Chien-chih

  Institute Of Biochemistry National Yangming University

• Chen S‐k

  National Yang Ming Univ. Medical School Taipei Twn

• CHEN SHIN-KUANG

  Institute of Environmental Health Sciences, National Yangming University

• HSIEH WANHUA

  Institute of Environmental Health Sciences, National Yangming University

• TSAI MONG-HSUN

  Institute of Environmental Health Sciences, National Yangming University

• HONG ANTONIO

  Insitute of Aboriginal Health, Tzu Chi University

• Hsieh W

  National Yang Ming Univ. Medical School Taipei Twn

• Hong Antonio

  Insitute Of Aboriginal Health Tzu Chi University

• Tsai M‐h

  National Yang Ming Univ. Medical School Taipei Twn

• Wei Yau-huei

  Institute Of Biochemistry And Molecular Biology National Yang-ming University

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