反射スペクトル分析装置の基礎的検討 : In-vitroにおけるキャリブレーションテスト

概要

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Spectrophotometry is a non-invasive, optical technique frequently used for the objective measurement of skin redness providing information of hemoglobin content and oxygen saturation in the skin. The accuracy of results and/or its interpretation relies on appropriate calibration methods. Purposes : 1) to determine whether the tarameters of hemoglobin content (IHB) and blood oxygenation (IOX) are proportionally changed when the hematocrit and oxygen content in blood are changed. 2) to verify that these parameters change independently of each other. Methods : The freshly sampled human blood in phosphate buffered saline was circulated through the calibration chamber where the probe of spectrophotometer was placed. The parameters of IHB and IOX were monitored when oxygen content in the blood solution was changed using O_2 certified mixed gas to establish different deoxy- and oxy-hemoglobin. The raw spectrum data processed in the instrument was sent to a computer to calculate IHB and IOX based on Feather's study. Results : The positive relationship was found between IHB and hematocrit (r=0.999). The value of IHB was not affected by changes in oxygen content in blood. The value of IOX was increased with increasing oxygen content in blood, and was not changed when the hematocrit was changed.
日本医療機器学会の論文
1994-07-01