異種移植における抗体の研究
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概要
- 論文の詳細を見る
Since Medawar et al.(1954)demonstrated adoptively acquired immunity in allogeneic transplantation by living sensitized lymphoid cells, many investigators have confirmed that cell-bound antibody is responsible for allograft rejection reaction and for delayed-type hypersensitivity in allograft immunity. Serum antibody, on the contrary, has generally been accepted to play a major role in xenogeneic transplantation. Although participation of cell-bound antibody has not been considered in xenograft immunity, it appears to be valuable for understanding the mechanism functioning in delayed-type reaction to investigate cell-bound antibody as well as humoral antibody in xenograft immunity. In guinea pigs transplanted with skin xenografts from C57BL mouse, delayed-type skin reaction was evoked by intradermal injection of C57BL spleen cell extract 7 to 10 days after the 1st-set grafting. Then, immediate-type reaction waso bserved 10 days after the 2nd-, and 3rd-set grafting. In the 3rd-set xenografting that revealed immediate-type skin reaction, precipitating antibody in the serum was demonstrated in capillary method and in double gel diffusion. The antibody was identified as IgG on immunoelectrophoretic analysis. In the 1st-set grafting that revealed delayed-type skin reaction, low levels of antibody activities in cytotoxic test and IAHA (immune-adherence hemagglutination) were shown in the serum. But no precipitating antibody was detected. By the method of antibody dissociation with hypertonic saline, antibody-like globulin was obtained from washed lymph node cells of the guinea pigs immunized with 1st-set xenograft. This globulin showed flocculation reaction instead of precipitation against C57BL tissue extract, and the antibody was identified as IgM on immunoelectrophoresis. Such as antibody was absorbed with a large amount of allogeneic gunea pig node cells. The evidences obtained in these experiments indicated that mechanism of delayed-type reaction functions in the early stage of xenograft immunity and the cell-bound antibody would be a cross-reacting antibody as proposed by Nelson, R. A., Jr. and demonstrated by Fujii et al. in allograft immunity in mice.
- 一般社団法人日本アレルギー学会の論文
- 1968-05-30