溶連菌に関する研究 : 第1報. Streptococcal DPNase および antistreptococcal DPNase について

元データ 1967-07-30 日本アレルギー学会

概要

The measurement of ASLO has become a useful means in the diagnosis and etiological consideration of the various diseases. It is known that many other exotoxins besides ASLO are produced by the hemolytic streptococcus, although investigations of these substances are not sufficient. Studies on the role of these toxic substances in allergic conditions as a consequence of hemolytic streptococcal infections are very few. In view of these facts the author investigated the specific antibodies against DPNase, one of the important exotoxins, in patients with various diseases, and performed the experiments of sensitization with exotoxins of hemolytic streptococcus. The summary of the results is as follows. 1) DPNase is heat labile; its opotimum temperature is 37℃ and the pH is 7.4, although cholesterol exhibits an inhibitory action on streptolysin activity, it does not inhibit DPNase activiy. 2) It is possible to isolate DPNase from SLO with DEAE cellulose column chromatography. 3) The ASDA value (anti DPNase) is high in rheumatic fever and acute nephritis; the average value is respectively and 460 units, which is definitely higher than the values in other diseases. The ASDA value showed a low titer of 12 units, on the average, in rheumatic arthritis. 4) The measurement of the ASDA together with the ASLO is a valuable method in the detection of previous infections with hemolytic streptooccus. Shince only one of the two tests may show a higher value, the diagnostic efficacy of the method is enhanced by employing both determinations at the some time. The ASDA test is particularly advantageous in clinical practice, because it does not show a false positive reaction in hypercholsterolemia.

著者

鳥越 公造 昭和大学医学部第1内科

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