呼吸虫皮内反応抗原物質に関する研究
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概要
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Skin tests were conducted on patients with antigens fractionated by Colum chromatography and the-following results were obtained. 1) CM-Cellulose chromatography of the crude antigen was conducted and 6 antigens such as CM1, CM2, CM3, CM4, CM5, and CM6 were obtained. Among these antigens, antigen CM1 produced the most remarkable reaction on paragonimiasis patients and showed no reaction in healthy individuals. The chemical components of the 6 antigens were analyzed by coleman spectrophotometor and chemical reactions. Antigen CM1 contained 235, 000γ protein per ml and 12, 000γ sugar per ml. Antigen CM2, CM3, CM4, CM5, and CM6 contained also less amount of protein and sugar than antigen CM1. 2) Antigen CM1 was further fractionated by DEAE-Cellulose chromatography and 7 fractions such as antigens A1, A2, B, C1, C2, D and E were obtained. Intradermal tests with these 7 antigens showed that antigen C1 provoked the most noticeable reaction by the injection of 0.0001γ of antigenic material on paragonimiasis patients. The chemical components of each fraction of these 7 antigens were analyzed. Antigen A1 and A2 contained a relatively large amounts of sugar, whereas antigen C1 and C2 contained a large amount of protein and a small amount of nucleic acid. 3) Antigen C1S and C1P were prepared by the centrifugation (100, 000 xg, 60min.) from antigen C1. Antigen C1S produced remarkable reaction by the injection of 0.0001γ of antigenic material in amounts of 0.02ml. Antigen C1S showed less remarkable reaction in individuals infected with or without other helminths than P. westermani. Antigen C1S contained 86.7γ protein per ml. It is considered that this protein was the most responsible components for the skin reaction. Conclusively, it is understood that the antigen C1 and C1S could be employed for the diagnosis of Paragonimiasis as they showed a remarkable reaction on Paragonimiasis patients and showed on reaction on heatlhy individuals with other helminth than P. westermani.
- 一般社団法人日本アレルギー学会の論文
- 1963-09-30