4. コーチゾンで遊離される蛋白質水解酵素 (Cortisone-Released Protease Preparation:CRPP) によるプラディキニンの分解

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概要

• 論文の詳細を見る

• Previously, Gladner et al. reported that normal rats contain no proteolytic or free collagenolytic activity (J.C. Houck, Y.M. Patel, J. Gladner: Biochem. Pharmacol., 16, 1099, '67). Within 26 hrs after administration of cortisol, prednisolone, indomethacin, or oxyphenylbutazone, this cutaneous compartment demonstrated both a proteolytic activity and a free collagenolytic activity. The enzyme with the above activities was named by Gladner et al. as "CRPP" (Cortisone-Released Protease Preparation). Salicylate administration to rats did not produce these results. Furthermore it was found that neither the proteolytic activity (maximal at pH 7.5) nor the collagenolytic activity (maximal at pH 5.5) was derived from the breakdown of intracellular lysosomes. We found that incubation of bradykinin with CRPP causes rapid and irreversible destruction of the smooth muscle contiaction activity of this compound. This activity was completely inhibited by diisopropyl-fluorophosphate (DFP). Then, the action of "CRPP" on bradykinin was examined and the hydrolytic site was demonstrated. Bradykinin (2.5 mg) was dissolved in 2 ml of M/15 sodium phosphate buffer, pH 7.5, the solution was mixed with 20mg of "CRPP" and was incubated for 20 hr. at 37c. After addition of 0.1 ml of glacial acetic acid the solution was evaporated to dryness at room temperature. The residue had no action upon isolated smooth muscle preparations. The residue was submitted to high voltage paper electrophoresis on Whatman No. 3 MM paper. The products isolated were applied to amino acid analysis. From the results it was demonstrated that bradykinin was hydrolyzed in the linkage expressed in arrows by the action of "CRPP". H. Arg-Pro-Pro-Gly-Phe-Ser-pro-Phe-Arg. OH

• 日本アレルギー学会の論文
• 1969-06-30

著者

• 鈴木 友二

  阪大蚤白研

• 鈴木 友二

  阪大蛋白研

• 林 恭三

  阪大蛋白研

• Gladner J

  National Insitite of Arthritis and Metabolic Diseases, National Institutes of Health

• Gladner J

  National Insitite Of Arthritis And Metabolic Diseases National Institutes Of Health

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• 4. コーチゾンで遊離される蛋白質水解酵素 (Cortisone-Released Protease Preparation:CRPP) によるプラディキニンの分解
• 14.蛇毒中のNicotinamide adenine diphosphataseについて(第179回会議研究発表要旨)
• Synthesis of Quinoline Derivatives of Amino Acid.

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