結核菌免疫マウスに於ける血中抗体の研究

概要

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Fifteen groups of mice (ddN, CF#1, CFW and gpc) were immunized by intraperitoneal, intramuscular or intravenous injection of the heat killed tubercle bacilli (H_<37>RV) or the BCG vaccine. Pooled sera were prepared by cardial puncture from five mice at weekly intervals. Serum sample were examined by Middlebrook-Dubos' passive hemagglutination, conditioned hemolysis and Sindo-Utahashi's modified Coombs' test using tuberculin fractions as the antigens. It was proved that production of specific antibodies against tubercle bacilli in mice, in general, was very low. In cases of immunized mice with the heat killed organisms, no antibodies could be found by those testings in vitro. But serum samples obtained from mice immunized with BCG at the 7-9th week were proved to be definitely positive in the hemolytic reaction and the modified Coombs' test in which the incomplete antibodies are thought to participate. No bivalent antibodies, as a whole, could be detected by those methods under given conditions. Amongst several sorts of mice, CF#1 is considered to be most sensitive and homogeneous in production of antibodies against tubercle bacilli.
日本アレルギー学会の論文
1960-10-01