Construction of fibrillar matrix for the culture of multiple renal glomerular cells

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概要

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• The mimic enviroment to culture renal glomerular cells such as epithelial cells, endothelial cells or mesangial cells requires the basement -membrane -like scaffold. Moreover, epithelial cells is required to be cultured at the opposite site of the basement membrane. In order to confirm if the in vivo environment could be constructed in vitro, we firstly constructed immortalized normal cells of mesangial and epithelial cells, and then co-cultured both cells at the opposite sites of a fibrillar gauge-gel. The polarity of epithelial cells was observed to evaluate if the constructed fibrillar matrix is suitable to be the scaffold for multiple glomerular cells.

• 一般社団法人日本機械学会の論文

著者

• Wang Pi

  Institute Of Applied Biochemistry University Of Tsukuba

• Wang Pi

  Institute Of Applied Biochemistry Tsukuba University

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