Encapsulation and culture of chondrocytes in collagenglycosaminoglycan composite matrices
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概要
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In order to regenerate cartilage tissue using tissue engineering technology, synthetic or naturally-derived polymers have been used as a scaffold. The naturally-derived polymers have been often cross-linked with chemical agents to obtain water-insoluble matrices. In the present study, we focused the cross-linking of collagen and glycosaminoglycans (GAG) with the poly (ethylene glycol) four-armed star polymer having active ester groups, i.e. (pentaerythritol poly (ethylene glycol) ether tetrasuccinimidyl glutarate). The gel matrices of collagen and collagen-GAG composite were successfully synthesized by the four-armed star polymer of 0.3-1mM, and the gel-formation time could be controlled by changing its reaction temperature from 4℃ to 37℃ under physiological pH and salt concentration. Chondrocytes were homogeneously encapsulated into the collagen-GAG composite gel, and kept their original round shape. After a week culture, safranin-O-stained revealed the biosynthesis of negatively-charged proteoglycans around the chondrocytes.
- 一般社団法人日本機械学会の論文
著者
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MATSUMOTO H.
Department of Physics, University of Alberta
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Tanaka J.
Biomaterials Center, NIMS
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Toyama Y.
Department Of Anatomy And Developmental Biology Graduate School Of Medicine Chiba University
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Toyama Y.
Department Of Orthopaedic Surgery School Of Medicine Keio University
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Taguchi T.
Biomaterials Center, National Institute for Materials Science
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Kobayashi H.
Biomaterials Center, National Institute for Materials Science
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Kataoka K.
Biomaterials Center, National Institute for Materials Science
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Kubo K.
NOF corporation
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Kitano S.
NOF corporation
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Matsumoto H.
Department of Applied Physics, Seikei University:(Present address)Institute of Physics, Univ. of Tsukuba
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