A New Image Analysis System for Biological Dosimetry by Fluorescent in situ Hybridization. Step 1: Metaphase Finder and Automatic Metaphase Acquisition Validation
スポンサーリンク
概要
- 論文の詳細を見る
Because of the large number of cells to be analyzed in cases of overexposure to ionizing radiation, an automated imaging system is desirable for scoring both translocations and dicentrics. This system should include three essential steps: automatic metaphase finding, automatic image capture at high magnification, and, finally, optimized data analysis for aberration interpretation. We evaluated a new image analysis system (CYTOGEN, IMSTAR, France) and found that its metaphase finder saved time, as much as quadrupling the speed of scoring chromosomal aberrations. Automatic metaphase selection did not appear to induce bias. We confirmed the equivalence of observing aberrations on a screen after automatic image capture and direct observation under a microscope. This work validated all of the steps necessary for obtaining images for automatic chromosomal aberration detection. The protocols for the detection of translocations may now be applied for biological dosimetry. This step will be validated in a future study.
- 日本放射線影響学会の論文
著者
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Voisin Philippe
Institut De Radioprotection Et De Surete Nucleaire
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Delbos Martine
Institut De Radioprotection Et De Surete Nucleaire
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Roy Laurence
Institut De Radioprotection Et De Surete Nucleaire
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Durand Valerie
Institut De Protection Et De Surete Nucleaire Departement De Protection De La Sante De L'homme
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SOROKINE-DURM IRENE
Institut de Protection et de Surete Nucleaire, Departement de Protection de la sante de l'Homme et d
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SOUSSALINE FRANCOISE
IMSTAR
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Sorokine-durm Irene
Institut De Protection Et De Surete Nucleaire Departement De Protection De La Sante De L'homme
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- A New Image Analysis System for Biological Dosimetry by Fluorescent in situ Hybridization. Step 1: Metaphase Finder and Automatic Metaphase Acquisition Validation