Ionizing Radiation and Bacterial Challenge Alter Splenic Cytokine Gene Expression

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概要

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• Irradiation increases susceptibility to bacterial infection. Exogenous proinflammatory cytokines can alter the response of mice to γradiation, but the role of endogenous inflammatory cytokines after bacterial infection in irradiated animals is not known. Gene expression of hematopoietic (GM-CSF) and proinflammatory (IL-1β, IL-6 and TNF-α ) cytokines were examined in spleens of B6D2F1/J female mice after irradiation alone (1.0- and 7.0-Gy), and after irradiation followed by Klebsiella pneumoniae s.c. challenge 4 days postirradiation by using the reverse transcription-polymerase chain reaction (RTPCR) and Southern blot hybridization. At 4, 8, and 24 h after bacterial challenge in 7.0-Gy-irradiated mice, GM-CSF mRNA increased (p < 0.05). TNF-αmRNA in irradiated mice were slightly decreased, whereas after bacterial challenge, TNF-αmRNA elevated at 30 h in 7.0-Gy-irradiated mice; at 4, and 8 h in 1.0-Gy-irradiated mice, and at 1 h in sham-irradiated mice (p < 0.05). IL-6 mRNA displayed a biphasic response in 7.0-Gy-irradiated mice, and, after bacterial challenge, in both irradiated mice (1.0- and 7.0-Gy) and sham-irradiated mice. IL-1βmRNA remained at or below normal for 8 h and increased at 24 h after bacterial challenge on day 4 in 7.0-Gy-irradiated mice. These results indicate that sublethal gamma radiation alters the patterns of the hematopoietic and proinflammatory cytokine responses to bacterial challenge in vivo. Consequently, treatment protocols may need to take into account changes in cytokine gene responses to resolve infection after irradiation.

• 日本放射線影響学会の論文

著者

• Jackson W

  Armed Forces Radiobiology Res. Inst. Md Usa

• Dobson M.e.

  (present Address) Division Of Microbiology Armed Forces Institute Of Pathology

• Chang C.m.

  Radiation Medicine Department Armed Forces Radiobiology Research Institute

• ELLIOTT T.B.

  Radiation Medicine Department, Armed Forces Radiobiology Research Institute

• JACKSON W.E.

  Computer and Electronics Division, Armed Forces Radiobiology Research Institute

• LEDNEY G.D.

  Radiation Medicine Department, Armed Forces Radiobiology Research Institute

• Elliott T.b.

  Radiation Medicine Department Armed Forces Radiobiology Research Institute

• Ledney G.d.

  Radiation Medicine Department Armed Forces Radiobiology Research Institute

• Chang C.

  Radiation Medicine Department, Armed Forces Radiobiology Research Institute

• Elliott T.

  Radiation Medicine Department, Armed Forces Radiobiology Research Institute

• Dobson M.

  (Present Address) Division of Microbiology, Armed Forces Institute of Pathology

関連論文

• Ionizing Radiation and Bacterial Challenge Alter Splenic Cytokine Gene Expression

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