Structural Chromosome Analysis by Whole Chromosome Painting for Assessment of Radiation-Induced Genetic Damage

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概要

• 論文の詳細を見る

• Accurate assessment of radiation exposure is important for several purposes including: 1) Detection of occupational or environmental exposure for regulatory purposes, 2) Evaluation of the level of accidental exposure to guide medical treatment, 3) Assessment of the level of exposure in populations in order to understand the long term biomedical consequences of exposure and 4) Measurement of the radiosensitivity of tumors and normal tissues in order to optimize cancer therapy. Physical dosimetry is frequently not available or unhelpful in these situations so that biological indicators of exposure are needed. One widely used indicator of radiation exposure is the frequency of structural chromosome aberrations in peripheral lymphocytes. Numerous studies during the past three decades have shown that the frequency of such aberrations increases with dose in a well defined manner (reviewed in 1) and thus can be used as an indication of exposure. The frequency of dicentric chromosomes is measured most often in these assays since these aberrations can be scored rapidly and accurately. This allows sufficient cells (often several hundred to a thousand) to be scored so that statistically precise estimates of the aberration frequency can be made. This is especially important when studying low exposures since the frequency of aberrations may be only a few per thousand cells. Dicentric frequency assays have proved to be most useful for assessment of dose in individuals exposed to acute, low LET radiation immediately after irradiation. Indeed, dicentric frequency analysis is the method of choice for dose estimation in such situations. However, estimation of dose from the dicentric chromosome frequency is much more difficult when the exposure is chronic or when the analysis is carried out at long times after irradiation because dicentrics are lost during cell division. Dosimetry based on the frequency of stable aberrations such as translocations is preferable in these cases since transloca-tions are retained in peripheral lymphocytes more-or-less indefinitely. However, estimation of translocation frequencies using conventional staining or banding analysis has been too labor intensive and time consuming to be routinely practical. As a result, translocation frequency analysis has been carried out only in a few studies.

• 日本放射線影響学会の論文

著者

• GRAY Joe

  Division of Molecular Cytometory, Department of Laboratory Medicine, University of California

• Awa Akio

  Radiation Effects Research Foundation

• Pinkel Daniel

  Division Of Molecular Cytometry Department Of Laboratory Medicine University Of California

• Gray Joe

  Division Of Molecular Cytometory Department Of Laboratory Medicine University Of California

• LUCAS JOE

  Biomedical Sciences Division, Lawrence Livermore National Laboratory

• Lucas Joe

  Biomedical Sciences Division Lawrence Livermore National Laboratory

関連論文

• 蛍光色素直接標識プローブによる迅速間期細胞遺伝学的診断
• Chromosome aberration : Inv(14)(q11q32) in lymphocytes from Hiroshima A-bomb survivors
• T-lymphocyte Stem Cell Structure Deduced from Clonal Chromosome Aberrations
• Analysis on translocation frequencies in Hiroshima A-bomb survivors by fluorescence in suu hybridization.
• Structural Chromosome Analysis by Whole Chromosome Painting for Assessment of Radiation-Induced Genetic Damage
• The Children of Parents Exposed to Atomic Bombs: Estimates of the Genetic Doubling Dose of Radiation for Humans : II. BIOLOGICAL EFFECTS
• Molecular cytogenetics and the assessment of canser risk

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