DNA Repair Characteristics of a Hybrid Cell Clone between Xeroderma Pigmentosum and Potorous tridactilis

スポンサーリンク

概要

• 論文の詳細を見る

• A hybrid cell clone PX1 was isolated by fusing UV sensitive XP20S(SV)neo, an SV-40-transformed, neomycin-resistant xeroderma pigmentosum (XP) cell line, and Pt K2, a rat kangaroo (Potorous tridactilis) cell line. The UV-survival curve of PX1 cells fell midway between those of Pt K2 and XP20S(SV)neo cells, since mean lethal doses(D_0) were 2.5, 4.7 and 0.27 J/m^2 for PX1, Pt K2 and XP20S(SV)neo, respectively. Amounts of unscheduled DNA synthesis (UDS) after UV, relative to normal human cells, were 60.4% for Pt K2, 37.7% for PX1 and 0.1% for XP20S(SV)neo. Such relative UDS capacities for excision repair of Pt K2, PX1 and XP20S(SV)neo were also consistent with the respective relative capacities of host cell reactivation (HCR) of UV-irradiated Herpes simplex virus. Apparently, there was no single Pt K2 chromosome in the PX1 cells. One possibility is that a gene which may account for the partial restoration of the UV resistance has been transferred from Pt K2 to PX1.

• 日本放射線影響学会の論文

著者

• Ida Kenji

  Department Of Experimental Radiology And Department Of Gynecology And Obstetrics Faculty Of Medicine

関連論文

• DNA Repair Characteristics of a Hybrid Cell Clone between Xeroderma Pigmentosum and Potorous tridactilis

スポンサーリンク